MERRELL outlet的問題,透過圖書和論文來找解法和答案更準確安心。 我們找到下列包括賽程、直播線上看和比分戰績懶人包
另外網站Merrell - The Outlet Shops of Grand River也說明:Merrell Outlet carries a complete selection of seasonal footwear and clothing for men and women. The store's design reinforces Merrell's dedication to ...
國立臺灣海洋大學 食品科學系 陳泰源、黃登福所指導 郭書豪的 利用二維電泳結合串聯式質譜儀研究酸性、微酸性電解水對腸炎弧菌之殺菌機制 (2011),提出MERRELL outlet關鍵因素是什麼,來自於二維電泳、酸性電解水、微酸性電解水、腸炎弧菌、串聯式質譜儀。
最後網站銷售據點| MERRELL台灣官網則補充:MERRELL 民生門市12:30~21:00 · MERRELL 林口三井OUTLET · MERRELL 三創生活3F · MERRELL 新光三越台北信義新天地A11 · MERRELL 新光三越南西1館8F · MERRELL 忠孝SOGO 10F 專櫃.
利用二維電泳結合串聯式質譜儀研究酸性、微酸性電解水對腸炎弧菌之殺菌機制
為了解決MERRELL outlet 的問題,作者郭書豪 這樣論述:
腸炎弧菌為食品安全上重要的公共衛生問題,已有許多文獻證實酸性電解水 (acidic electrolyzed water, AEW) 可做為殺菌劑應用在食品,但其可能腐蝕食品加工器具和管路。相反地,微酸性電解水 (slightly acidic electrolyzed water, SlAEW) 較 AEW 在使用上安全。本研究中所使用之試樣溶液為含有 80 mg/L 氯濃度的 SlAEW、AEW 和 NaOCl,磷酸緩衝鹽 (phosphate-buffered saline, PBS) 為控制組,在殺菌能力及萃取蛋白實驗中各試樣溶液之物理、化學特性可保持穩定。以 SlAEW 處理腸炎弧
菌 180 秒,可使腸炎弧菌數量由 8.12 log CFU/mL 下降至平板計數法無法計數出,與 AEW 和 NaOCl 效果相當。同時使用 PBS 處理腸炎弧菌 180 秒,發現其不會對腸炎弧菌的數量造成太大的影響。本研究中以SlAEW、AEW 和 NaOCl 處理腸炎弧菌後,比 PBS組之蛋白質濃度分別下降了 31.5、88.2 和 61.3%,由 SDS-PAGE 發現經試樣溶液處理後,圖譜確實有所變化,且發現蛋白質亮帶有消失的現象,顯示試樣溶液會破壞腸炎弧菌蛋白質,相較之下 SlAEW 破壞蛋白質的情形較輕微。以二維電泳 (two-dimensional electrophoresi
s, 2DE) 分析腸炎弧菌整體蛋白質後,選取表現量明顯上調和下調的蛋白質點利用 MALDI-Q-TOF 及 LC/MS/MS 進行蛋白質定性,結果共比對到 7 個蛋白質,經過次氯酸鈉處理後表現量上調的 Outer membrane protein OmpK,為弧菌噬菌體 KVP40 的接受器;表現量下調的 Adenylate kinase、Phosphoglycerate kinase、Glyceraldehyde-3-phosphate dehydrogenase A 和 Enolase,和能量代謝相關;表現量下調的 Glyceraldehyde-3-phosphate dehydroge
nase A 和 Elongation factor Tu,和蛋白質合成相關;表現量上調的 Outer membrane protein U,和滲透壓調節相關。本論文證實,腸炎弧菌經過微酸性電解水、酸性電解水和次氯酸鈉處理後,可抑制 ATP 及蛋白質合成相關之蛋白,並對細菌造成無法負荷之滲透壓,使菌體死亡。
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