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Let’s Make a Pasta Salad

為了解決Side dish的問題，作者Bolte, Mari 這樣論述：

Pasta comes in all shapes, sizes, colors, and flavors. Starch and proteins work together to create the firm texture that makes a great bowl of pasta. Learn how to make your own pasta salad. Serve it as a summery side dish or a full, filling meal! Includes a glossary, list of sources for further r

eading, and a recipe with step-by-step instructions.

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探討克雷白氏肺炎桿菌CG43之大毒力質體pLVPK對第三型纖毛表現的影響

為了解決Side dish的問題，作者江柏毅 這樣論述：

克雷白氏肺炎桿菌CG43是一分離自糖尿病患肝膿瘍的菌株，帶有一大毒力質體pLVPK。第三型纖毛是克雷白氏肺炎桿菌主要的黏附因子，也是決定其形成生物膜的必要因子，已知Fur-Fe+2、c-di-GMP及H-NS可正向調控第三型纖毛的表現。除了莢膜多醣生成的調控蛋白基因rmpA及rmpA2，攝鐵系統基因iucABCD-iutA、iroBCDN、fepBC及fecIRA外，pLVPK還帶有可轉譯為c-di-GMP環化酶的LV144及H-NS同源基因hnsp。比較剔除pLVPK後的CG43101和CG43S3101與親本菌株CG43和CG43S3的表現型發現：CG43101和CG43S3101的多醣

莢膜和螯鐵分子的生成量明顯較低，而胞外多醣生成量、抗酸能力沒有明顯改變；相對的，第三型纖毛單位蛋白MrkA及生物膜生成量明顯提升，此顯示pLVPK可能扮演負向調控第三型纖毛表現的角色。為了找出此負向調控的決定因子，首先將pRK415-rmpA及pRK415-rmpA2分別轉型入CG43S3101，結果發現可明顯提升其莢膜多醣生成量，但對MrkA或生物膜的生成量都沒有明顯影響；而比較CG43S3101fur、CG43S3101ryhB和CG43S3101furryhB與CG43S3fur、CG43S3ryhB、CG43S3furryhB後發現：剔除pLVPK使Fur缺乏鐵的活化而

失去抑制小RNA RyhB能力；再比較CG43S3101[pRK415-hnsp]和CG43S3101[pRK415]，結果顯示增加hnsp表現可降低MrkA和生物膜的生成量；最後比較CG43S3101[pRK415-LV144] 與CG43S3101[pRK415]發現LV144的表現也可降低MrkA和生物膜的生成量。綜合上述結果顯示：莢膜多醣生成或Fur-RyhB調控與pLVPK負向影響第三型纖毛的表現可能無關，而H-NSp和LV144可能為pLVPK負向調控第三型纖毛表現的決定因子，其調控機制將待進一步探討。

Healthy Mediterranean Diet Cookbook: 50+ Portioned Recipes for Healthy Eating

為了解決Side dish的問題，作者Barnes, Gloria 這樣論述：

If you’re looking for a diet that will keep your body lean and healthy, and your taste buds happy, the Mediterranean Diet is worth considering. The Mediterranean Diet is rich in plant-based proteins, heart-healthy fats, antioxidants and fiber. In contrast with many popular diets out there today whic

h restrict certain food groups or emphasize processed foods as their main source of protein, the Mediterranean Diet is all about enjoying what nature has to offer, both inside and outside the kitchen. The diet’s emphasis on seasonal produce also means that you’ll get vital nutrients at their peak ri

peness when they’re most easily absorbed by our bodies - promoting weight loss and increased energy levels. Finally, the diet also includes beans, seeds and nuts, as well as healthy oils like olive oil, which provides a huge amount of omega-3 fatty acids - a type of ’healthy fat’ found in certain fo

ods that’s been linked to a whole range of health benefits. This book covers ? Breakfast recipes ? Appetizers and snacks ? Main dish ? Vegetables and side dishes ? Soup and stew recipes And much more The Mediterranean Diet can be tracked back to the traditional diets of people living in Greece and S

outhern Italy, where they consume plenty of fish and fresh produce. It has also been described as a ?blueprint for well-being? by the WHO (World Health Organization) because it is low in saturated fats ? something which is common with many modern diets today but highly detrimental to our overall hea

lth. When we consume these foods, they’re broken down into their essential vitamins and minerals by the body - creating an easily absorbed source of fuel. In contrast, processed foods don’t contain much fiber or protein; they’ve lost much of their nutritional value through processing. The Mediterran

ean Diet is rich in plant-based proteins (like legumes, grains, nuts and seeds) as well as fish and seafood.

The Biological Effects of Photobiomodulation on Human Adipose Mesenchymal Stem Cells and Its Potential in Cell Therapy

為了解決Side dish的問題，作者NGUYEN LE THANH HANG 這樣論述：

Background: Photobiomodulation (PBM) of human adipose-derived mesenchymal stem cells (hADSCs) using near-infrared (NIR) laser irradiation has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration.Resea

rch purposes: A proposed single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were established for investigating the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human a

dipose mesenchymal stem cells (hADSCs). In addition, the hADSC migration assay was examined at an optimal fluency to interpret the correlation between the PBM-induced increase in ΔΨm, ROS, vesicle transport, and cell migration.Materials and Methods: The hADSCs were first seeded into a flow chamber a

nd incubated for 24 hours. Rhodamine 123 (Rh123) and 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) were used to perform the ΔΨm and ROS stimulation on irradiated-hADSCs , respectively, with irradiation (830 nm) in the range of 2.5-10 (J/cm2), where time series of bright-field images were also

obtained to identify the vesicle transport phenomenon. In addition, the PBM effect in stimulating the irradiated-hADSC movement at an optimal fluency (1.6 J/cm2) was investigated using culture-insert two wells in a 35 mm dish.Results: Present results revealed that a fluence of 5 J/cm2 significantly

promoted the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm2) ) at 30 mins-post irradiation . Furthermore, the improvement in hADSC migration was statistically indicated between the PBM group (5 J/cm2) and the control group (0 J/cm2 + 10% FBS) at 24 hours post-irrad

iation.Conclusion: Consequently, our single-cell research had demonstrated an increase of the ΔΨm, ROS, and vesicle transport at a fluence of 5 J/cm2 in the irradiated-hADSCs. Moreover, an improvement in hADSC movement was observed at an optimal fluency of 1.6 J/cm2. These data indicated the usage

of PBM can to stimulate positive biological effects which results in stimulating cell proliferation, migration, and differentiation in stem cell-based therapy.