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American Moonshot: John F. Kennedy and the Great Space Race

為了解決Giant Propel的問題，作者Brinkley, Douglas 這樣論述：

Instant New York Times BestsellerAs the fiftieth anniversary of the first lunar landing approaches, the award winning historian and perennial New York Times bestselling author takes a fresh look at the space program, President John F. Kennedy's inspiring challenge, and America's race to the moon."We

choose to go to the Moon in this decade and do the other things, not because they are easy, but because they are hard; because that goal will serve to organize and measure the best of our energies and skills, because that challenge is one that we are willing to accept, one we are unwilling to postp

one, and one we intend to win."--President John F. KennedyOn May 25, 1961, JFK made an astonishing announcement: his goal of putting a man on the moon by the end of the decade. In this engrossing, fast-paced epic, Douglas Brinkley returns to the 1960s to recreate one of the most exciting and ambitio

us achievements in the history of humankind. American Moonshot brings together the extraordinary political, cultural, and scientific factors that fueled the birth and development of NASA and the Mercury, Gemini and Apollo projects, which shot the United States to victory in the space race against th

e Soviet Union at the height of the Cold War. Drawing on new primary source material and major interviews with many of the surviving figures who were key to America's success, Brinkley brings this fascinating history to life as never before. American Moonshot is a portrait of the brilliant men and w

omen who made this giant leap possible, the technology that enabled us to propel men beyond earth's orbit to the moon and return them safely, and the geopolitical tensions that spurred Kennedy to commit himself fully to this audacious dream. Brinkley's ensemble cast of New Frontier characters includ

e rocketeer Wernher von Braun, astronaut John Glenn and space booster Lyndon Johnson.A vivid and enthralling chronicle of one of the most thrilling, hopeful, and turbulent eras in the nation's history, American Moonshot is an homage to scientific ingenuity, human curiosity, and the boundless America

n spirit.

Giant Propel進入發燒排行的影片

具熱響應之聚(N-異丙基丙烯醯胺)/瓊脂糖互穿式水凝膠的特性與應用

為了解決Giant Propel的問題，作者吳俊諺 這樣論述：

自1956年來聚(N-異丙基丙烯醯胺)在生醫領域的研究不斷地推陳出新，其受溫度刺激響應的特性也使對它的應用與研究跨足了其它如物理學、環境科學、能源等領域，聚(N-異丙基丙烯醯胺)是屬於一種具有可逆體積相變過程的材料，因為其結構上的功能性官能基團會受溫度高低而選擇與水分子或自身產生氫鍵，由此達到親疏水性轉換與發生體積相變。為了提高應用性，先前的研究將其合成互穿式高分子網路的水凝膠，同時保有溫敏性與加強機械性質或增加其他的響應模式。本研究開發出兩種不同型式的水凝膠，一種是用浸潤法使聚(N-異丙基丙烯醯胺)分子擴散進瓊脂糖的網路基底形成半互穿式高分子網路，另一種是採用紫外線誘導共聚合法合成出聚(N

-異丙基丙烯醯胺-co-聚乙二醇二甲基丙烯酸酯)與瓊脂糖的互穿式高分子網路水凝膠，兩種水凝膠都是將瓊脂糖與聚(N-異丙基丙烯醯胺)做結合，來開拓與以往不同的研究方向。在本研究中主要分為兩個部分，第一部分為研究了瓊脂糖基底的聚(N-異丙基丙烯醯胺)互穿式高分子網路水凝膠的性質，發現當聚(N-異丙基丙烯醯胺)會改變瓊脂糖水凝膠的溶脹特性，比起純的瓊脂糖水凝膠在溶脹平衡時可以保有更多水分。另外，聚(N-異丙基丙烯醯胺)也會改變透射度與機械性質，並且可以用溫度去操控其變化，我們提出了一個模型去解釋這個獨特的現象，當聚(N-異丙基丙烯醯胺)進入水凝膠網路時會因為氫鍵膠護作用而與瓊脂糖支架結合，加強其結構

並使折射率匹配，造成有較大的彈性模數與透射度變化，但當溫度高於聚(N-異丙基丙烯醯胺)的濁點時，聚(N-異丙基丙烯醯胺)會改變成與自身或同類分子產生氫鍵，進而改變自身的折射率，而與此同時因為與瓊脂糖網路分離，進而造成機械性質改變。第二部分為基於前一部分觀測的性質，設計三種不同的加熱方式與應用。第一種應用是基於通過溫度變化可逆地切換機械性能，以通過圖案化加熱系統生產具有不同局部機械強度的材料。第二種應用是智慧隔熱窗戶，它包含了一個可調節透射率的內層，其受紅外線加熱到相變溫度就會自動關閉，使室內不會接收過多的紅外線而升溫。第三種應用則是在水凝膠的表面輸送膠體粒子，當水凝膠表面的聚(N-異丙基丙烯醯

胺)分子相變時會將水分排出，故可利用紅外雷射掃描加熱水凝膠表面產生不對稱的流場，發現半互穿式高分子網路水凝膠與互穿式高分子網路水凝膠的產生的流場模式不同。並利用這種不對稱流場加以設計雷射掃描路徑製造出可以排開、聚集與單向輸送膠體粒子的表面流場，最後將材料做成微流道的基底，測試此材料輸送粒子的在微流道中的可行性。

American Moonshot: John F. Kennedy and the Great Space Race

為了解決Giant Propel的問題，作者Brinkley, Douglas/ Graybill, Stephen (NRT) 這樣論述：

As the fiftieth anniversary of the first lunar landing approaches, the award winning historian and perennial New York Times bestselling author takes a fresh look at the space program, President John F. Kennedy's inspiring challenge, and America's race to the moon."We choose to go to the Moon in this

decade and do the other things, not because they are easy, but because they are hard; because that goal will serve to organize and measure the best of our energies and skills, because that challenge is one that we are willing to accept, one we are unwilling to postpone, and one we intend to win."--

President John F. KennedyOn May 25, 1961, JFK made an astonishing announcement: his goal of putting a man on the moon by the end of the decade. In this engrossing, fast-paced epic, Douglas Brinkley returns to the 1960s to recreate one of the most exciting and ambitious achievements in the history of

humankind. American Moonshot brings together the extraordinary political, cultural, and scientific factors that fueled the birth and development of NASA and the Mercury, Gemini and Apollo projects, which shot the United States to victory in the space race against the Soviet Union at the height of t

he Cold War. Drawing on new primary source material and major interviews with many of the surviving figures who were key to America's success, Brinkley brings this fascinating history to life as never before. American Moonshot is a portrait of the brilliant men and women who made this giant leap pos

sible, the technology that enabled us to propel men beyond earth's orbit to the moon and return them safely, and the geopolitical tensions that spurred Kennedy to commit himself fully to this audacious dream. Brinkley's ensemble cast of New Frontier characters include rocketeer Wernher von Braun, as

tronaut John Glenn and space booster Lyndon Johnson.A vivid and enthralling chronicle of one of the most thrilling, hopeful, and turbulent eras in the nation's history, American Moonshot is an homage to scientific ingenuity, human curiosity, and the boundless American spirit.

鼻咽癌中高度甲基化的CDC20B

為了解決Giant Propel的問題，作者郭汶筑 這樣論述：

指導教授推薦書論文口試委員會審定書致謝 iii中文摘要 iv英文摘要 v目錄 vi圖目錄 ix表目錄 x第一章 前言 - 1 -1.1 鼻咽癌(Nasopharyngeal carcinoma, NPC)簡介 - 1 -1.2 Epstein -Barr Virus (EBV)簡介 - 2 -1.3 DNA甲基化簡介 - 2 -1.4 MicroRNA 449a (miR449a)簡介 - 3 -1.5 Cell division cycle protein 20 homolog B (CD

C20B)簡介 - 5 -1.6 Vimentin (VIM)簡介 - 6 -1.7 Tripartite motif-containing protein 29 (TRIM29)簡介 - 8 -1.8 Ubiquitination簡介 - 8 -第二章 研究目的 - 10 -實驗流程 - 11 -第三章 材料與方法 - 12 -3.1 細胞培養 (Cell culture) - 12 -3.2 VIM-2HA及CDC20B-3F不同功能區域之質體建構 - 12 -3.3 轉染作用 (Tran

sfection) - 13 -3.4 建立穩定表達shVIM的細胞株 - 14 -3.5 細胞生長試驗(Cell proliferation assay) - 14 -3.6 免疫共沉澱法(Co-immunoprecipitation, Co-IP) - 15 -3.7 西方墨點法(Western blot) - 16 -第四章 實驗結果 - 18 -4.1 miR449a/CDC20B的啟動子在鼻咽癌細胞中高度甲基化 - 18 -4.2 質譜儀分析CDC20B的交互作用蛋白 - 19 -4.3 CDC20B和VI

M的交互作用 - 20 -4.4 重新表達CDC20B可抑制鼻咽癌細胞增生 - 21 -4.5 VIM過度表現會促進細胞增生 - 22 -4.6 CDC20B影響鼻咽癌細胞內VIM的穩定性 - 23 -第五章 討論 - 25 -參考文獻 - 31 -圖表 - 36 -附錄圖表 - 46 - 圖目錄圖一、CDC20B和VIM之間的交互作用 - 36 -圖二、CDC20B過度表現會抑制細胞增生 - 37 -圖三、VIM過度表現會促進細胞生長 - 38 -圖四、降低VIM的表現量及同時送入CDC20B的影響

- 39 -圖五、CDC20B影響鼻咽癌細胞內VIM的穩定性 - 41 -圖六、建立不同片段的CDC20B-3F和VIM-2HA質體 - 42 -圖七、CDC20B在鼻咽癌中的分子機制模式圖 - 43 - 表目錄表一、Primers list - 44 -表二、VIM之shRNA - 45 -